BioChem - DOS

Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]

Moretti, Marcia M. S.; Bocchini-Martins, Daniela A.; Silva, Roberto Da; Rodrigues, André Sette, Lara D.; Gomes, Eleni

<P>Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45°C on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera <I>Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida</I>. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as <I>Aspergillus fumigatus</I> M.7.1 and <I>Myceliophthora thermophila</I> M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from <I>A. fumigatus</I> was stable from 40°C to 65°C and both endo-glucanase and xylanase from <I>M. thermophila</I> were stable in this temperature range when in absence of substrate. The enzymes were stable from pH 4.0 to 9.0.</P>

2012

Sociedade Brasileira de Microbiologia

cc-by-nc

1517-8382

1678-4405

43

3

pp.1062-1071

10.1590/s1517-83822012000300032

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74490629

http://www.scielo.br/pdf/bjm/v43n3/32.pdf

Thermophilic fungi; solid-state fermentation; xylanase; cellulase