BioChem - DOS

Pectinases immobilization on magnetic nanoparticles and their anti-fouling performance in a biocatalytic membrane reactor

RSC advances

Gebreyohannes, Abaynesh Yihdego; Mazzei, Rosalinda; Poerio, Teresa; Aimar, Pierre; Vankelecom, Ivo F. J.; Giorno, Lidietta

<P>Enzyme immobilization on commercial superparamagnetic nanoparticles (NP<SUP>SP</SUP>) was performed using covalent bonding. The biofunctionalized NP<SUP>SP</SUP> was then immobilized on the surface of the membrane using an external magnetic field to form a magneto-responsive biocatalytic membrane reactor (BMR<SUP>SP</SUP>). The magnetically formed smart nanolayer can be easily re-dispersed and recovered from the membrane when the enzyme is deactivated or whenever cleaning is required due to substrate over-accumulation. The system was used to hydrolyze pectin contained in different streams. Results are supported with complementary data from hydrodynamic, kinetic and morphological characterization in a flow-through reactive filtration. Wavelength-dispersive X-ray spectroscopy (WDS) elemental mapping revealed that the NP<SUP>SP</SUP> are uniformly dispersed on the surface of the membrane forming a thin biocatalytic layer. Both results of hydrodynamic studies and SEM micrographs of the membrane with the enzyme layer under various operating conditions, show that the immobilized enzyme effectively reduced membrane-foulant interaction. Comparison of filtration data using this commercial NP<SUP>SP</SUP> reveals good agreement with our previously used home-made NP<SUP>SP</SUP>. This implies that the scaling-up and commercialization of the developed BMR<SUP>SP</SUP> can be straightforward.</P>

2016

The Royal Society of Chemistry

cc-by-nc

2046-2069

6

101

pp.98737-98747

10.1039/c6ra20455d

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77498490

https://pubs.rsc.org/en/content/articlepdf/2016/ra/c6ra20455d