BioChem - DOS

Improved synthesis of disaccharides with Escherichia coli β-galactosidase using bio-solvents derived from glycerol

Tetrahedron

Pé rez-Sá nchez, Marí a; Corté s Cabrera, Á lvaro; Garcí a-Martí n, Hé ctor; Sinisterra, J. Vicent; Garcí a, José I.; Herná iz, Marí a J.

<P><B>Abstract</B></P><P>A noticeably increase in activity, keeping total regioselectivity was found in the synthetic behaviour of <I>Escherichia coli</I> &beta;-galactosidase in glycerol-based solvents using a 1:7 molar ratio of donor (<I>p</I>NP-&beta;-Gal): acceptor (GlcNAc). Yields of up to 97% of &beta;(1&rarr;6) with different solvents were found. These reactions take place without noticeable hydrolytic activity and with total regioselectivity, representing a considerable improvement over the use of aqueous buffer or conventional organic solvents. There is a clear dependence of the catalytic results on the solvent structure, which is analysed in terms of polarity and hydrophobicity.</P> <P><B>Graphical abstract</B></P><P><ce:figure id='dfig1'></ce:figure></P>

2011

0040-4020

1464-5416

67

40

pp.7708-7712

10.1016/j.tet.2011.08.009

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57315096

Biocatalysis; Solvent effects; Glycerol-derived solvents; Molecular dynamics; Transglycosylation