초록
<P><B>Abstract</B></P> <P>An application of nano-sized particles for an enhancement of biohydrogen production has gained global attention owing to their inherent potentials. In the current communication, influence of nickel oxide (NiO) and hematite (Fe<SUB>2</SUB>O<SUB>3</SUB>) nanoparticles (NP) on biohydrogen production have been investigated. The complex distillery wastewater was used as a substrate during batch fermentation test. Results showed that the maximum hydrogen yield (HY) and specific hydrogen production rate (SHPR) of 7.85 mmol/g COD and 16.71 mmol/g VSS.d, respectively, corresponding to sole addition of Fe<SUB>2</SUB>O<SUB>3</SUB> (200 mg/L) were approximately 1.2-fold higher than those NiO NP, and representing a 44%, and 196%, enhancement compared to control, respectively. The highest HY and SHPR of about 8.83 mmol/g COD, and 18.14 mmol/g VSS.d, respectively, were obtained for the batch test of complex distillery wastewater with co-addition of Fe<SUB>2</SUB>O<SUB>3</SUB> (200 mg/L) plus NiO NP (5 mg/L). The results of present study impeccably implies that the co-addition of Fe<SUB>2</SUB>O<SUB>3</SUB> and NiO NP is about 1.2–4.5 order more effective for enhanced hydrogen recovery from complex distillery wastewater compared to control, and sole nanoparticles addition. Furthermore, an enhanced activity of <I>ferredoxin oxidoreductase</I> and <I>hydrogenase</I> at Fe<SUB>2</SUB>O<SUB>3</SUB> plus NiO nanoparticles interface could be a plausible reason for an observed highest relative enhancement in HY, 62%, and SHPR, 221%, during batch test.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fe<SUB>2</SUB>O<SUB>3</SUB> and NiO nanoparticles for enhanced H<SUB>2</SUB> recovery from distillery waste. </LI> <LI> 44% increase in H<SUB>2</SUB> yield was obtained for sole hematite supplement. </LI> <LI> Co-addition of nanoparticles notably maximize H<SUB>2</SUB> yield compared to sole addition. </LI> <LI> Optimum concentration for co-addition of nanoparticles: 200 mg Fe<SUB>2</SUB>O<SUB>3</SUB>/L, 5 mg NiO/L. </LI> <LI> Relative enhancement in H<SUB>2</SUB> yield and rate at optimum concentrations: 62%, 221%. </LI> </UL> </P>