BioChem - DOS

Long-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes

Scientific reports

Liu, Guodong; Zhang, Lei; Qin, Yuqi; Zou, Gen; Li, Zhonghai; Yan, Xing; Wei, Xiaomin; Chen, Mei; Chen, Ling; Zheng, Kai; Zhang, Jun; Ma, Liang; Li, Jie; Liu, Rui; Xu, Hai; Bao, Xiaoming; Fang, Xu; Wang, Lushan; Zhong, Yaohua; Liu, Weifeng; Zheng, Huajun; Wang, Shengyue; Wang, Chengshu; Xun, Luying; Zhao, Guo-Ping; Wang, Tianhong; Zhou, Zhihua; Qu, Yinbo

<P>Long-term strain improvements through repeated mutagenesis and screening have generated a hyper-producer of cellulases and hemicellulases from <I>Penicillium decumbens</I> 114 which was isolated 30 years ago. Here, the genome of the hyper-producer <I>P. decumbens</I> JU-A10-T was sequenced and compared with that of the wild-type strain 114-2. Further, the transcriptomes and secretomes were compared between the strains. Selective hyper-production of cellulases and hemicellulases but not all the secreted proteins was observed in the mutant, making it a more specific producer of lignocellulolytic enzymes. Functional analysis identified that changes in several transcriptional regulatory elements played crucial roles in the cellulase hyper-producing characteristics of the mutant. Additionally, the mutant showed enhanced supply of amino acids and decreased synthesis of secondary metabolites compared with the wild-type. The results clearly point out that we can target gene regulators and promoters with minimal alterations of the genetic content but maximal effects in genetic engineering.</P>

2013

Nature Publishing Group

cc-by-nc-nd

2045-2322

3

pp.1569

10.1038/srep01569

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART80936276

https://www.nature.com/articles/srep01569.pdf