초록
Clostridium tyrobutyricum is well documented as a fermentation strain for the production of butyric acid. In this work, using high-energy carbon heavy ion irradiated C. tyrobutyricum, then butyric acid fermentation using glucose or alkali and acid pretreatments of Eucommia ulmoides Oliv. as a carbon source was carried out. Initially, the modes at pH 5.7-6.5 and 37<SUP>o</SUP>C were compared using a model medium containing glucose as a carbon source. When the 72gL<SUP>-1</SUP> glucose concentration was found to be the highest yield, the maximum butyric acid production from glucose increased significantly, from 24gL<SUP>-1</SUP> for the wild type strains to 37gL<SUP>-1</SUP> for the strain irradiated at 126AMeV and a dose of 35Gy and a 10<SUP>7</SUP>ions/pulse. By feeding 100gL<SUP>-1</SUP> acid pretreatments of E. ulmoides Oliv. into the fermentations, butyrate yields (5.8gL<SUP>-1</SUP>) and butyrate/acetate (B/A) ratio (4.32) were achieved.