<P><B>Abstract</B></P> <P>The production of sodium gluconate by enzymatic catalysis of delignified corn cob residue (DCCR) hydrolysate was studied. <I>Penicillium oxalicum</I> I1-13 was used for the production of cellulase with high β-glucosidase activity. A fed-batch saccharification process was developed to obtain high yields of glucose. At the end of hydrolysis, the concentration of glucose reached 145.80g/L. Glucose oxidase and catalase were co-immobilized to catalyze DCCR hydrolysate to produce sodium gluconate. Under the optimum conditions, 166.87g/L sodium gluconate was obtained after 56h of reaction, with a yield of 98.24%. The immobilized enzymes could still maintain more than 60% of the activity after repeated use for 6 times. This study provides a potential route for the production of valuable chemicals by enzymatic conversion of lignocellulosic materials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> On-site production of cellulase was performed for lignocellulose saccharification. </LI> <LI> 145.80g/L glucose was obtained by fed-batch saccharification of DCCR. </LI> <LI> 166.87g/L SG was produced by co-immobilized GOD-CAT under optimal conditions. </LI> <LI> The co-immobilized GOD-CAT could being reused at least 6 times. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>