초록
<P><B>Abstract</B></P> <P>In the present work, with corn stover hydrolysate as the substrate, an efficient hydrogen-producing thermophile, <I>Thermoanaerobacterium thermosaccharolyticum</I> W16, was added to three kinds of seed sludge (rotten corn stover (RCS), cow dung compost (CDC), and sludge from anaerobic digestion (SAD)) to investigate the effect of bioaugmentation on thermophilic hydrogen production. Batch test results indicate that the bioaugmentation with a small amount of the strain <I>T. thermosaccharolyticum</I> W16 (5% of total microbes) increased the hydrogen yield to varying degrees (RCS: from 8.78 to 9.90 mmol H<SUB>2</SUB>/g utilized sugar; CDC: from 8.18 to 8.42 mmol H<SUB>2</SUB>/g utilized sugar; SAD: from 8.55 to 9.17 mmol H<SUB>2</SUB>/g utilized sugar). The bioaugmentation process also influenced the soluble metabolites composition towards more acetate and less butyrate production for RCS, and more acetate and less ethanol accumulation for SAD. Microbial community analysis indicates that <I>Thermoanaerobacterium</I> spp. and <I>Clostridium</I> spp. dominated microbial community in all situations and might be mainly responsible for thermophilic hydrogen generation. For RCS and SAD, the bioaugmentation obviously increased the relative abundance of the strain <I>T. thermosaccharolyticum</I> W16 in microbial community, which might be the main reason for the improvement of hydrogen production in these cases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>T. thermosaccharolyticum</I> W16 raised thermophilic H<SUB>2</SUB> yield of corn stover hydrolysate. </LI> <LI> Bioaugmentation with W16 affected soluble metabolites towards more acetate production. </LI> <LI> <I>Thermoanaerobacterium</I> spp. and <I>Clostridium</I> spp. dominated the microbial community. </LI> <LI> Bioaugmentation increased the relative abundance of W16 in the microbial community. </LI> </UL> </P>