초록
<P><B>Abstract</B></P> <P>This work reports an improved process of enzymatic saccharification on <I>Agave salmiana</I> leaves and its conversion into bioethanol comparing strains of <I>Saccharomyces cerevisiae</I> ethanol RED and <I>Kluyveromyces marxianus</I> OFF1. <I>A. salmiana</I> leaves were pretreated with acid-alkaline sequential process before the application of several treatments of enzymatic saccharification. In the results, the best enzymatic treatment reached conversion of 94.49%, releasing sugar concentrations of 50 g L<SUP>−1</SUP>. During fermentation, glucose consumption efficiencies were similar for both strains with values of 98%, while the consumption rates were higher for <I>S. cerevisiae</I>. Fermentative efficiencies were higher for <I>K. marxianus</I> with values of 92.88 ± 3.24% while for <I>S. cerevisiae</I> they were 87.63 ± 2.23%. These findings show a saccharification enzymatic process efficiently applied on <I>A. salmiana</I> leaves. <I>K. marxianus,</I> a non-<I>Saccharomyces</I> strain, converted all the sugars released from this plant structure into bioethanol with similar values or even higher than a <I>Saccharomyces</I> strain.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enzymatic saccharification applied on <I>A. salmiana</I> leaves reached efficiencies of 94%. </LI> <LI> Enzymatic saccharification of <I>A. salmiana</I> leaves released up to 50 g L<SUP>−1</SUP> of sugars. </LI> <LI> Glucose consumption rates using <I>S. cerevisiae</I> were higher than using <I>K. marxianus</I>. </LI> <LI> The ethanol production rates were similar for both yeast strains. </LI> <LI> Fermentative efficiencies using <I>K. marxianus</I> were higher than using <I>S. cerevisiae</I>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>