초록
Butanol production by Clostridium acetobutylicum DSM 792 fermentation was investigated. Unsupplemented cheese whey was adopted as renewable feedstock. The conversion was successfully carried out in a biofilm packed bed reactor (PBR) for more than 3months. The PBR was a 4cm ID, 16cm high glass tube with a 8cm bed of 3mm Tygon rings, as carriers. It was operated at the dilution rate between 0.4h<SUP>-1</SUP> and 0.94h<SUP>-1</SUP>. The cheese whey conversion process was characterized in terms of metabolites production (butanol included), lactose conversion and biofilm mass. Under optimized conditions, the performances were: butanol productivity 2.66g/Lh, butanol concentration 4.93g/L, butanol yield 0.26g/g, butanol selectivity of the overall solvents production 82wt%.