BioChem - DOS

High robustness of a simplified microbial consortium producing hydrogen in long term operation of a biofilm fermentative reactor

International journal of hydrogen energy

Carrillo-Reyes, J.; Trably, E.; Bernet, N.; Latrille, E.; Razo-Flores, E.

Fixed-biomass reactors present the main advantage of increasing the hydrogen production rates by supporting high organic loading rates. However, the use of complex natural inoculum often precludes a long-term operation through the rapid emergence of methanogens. In this study, an Anaerobic Sequencing Batch Biofilm Reactor (ASBBR) was inoculated with a simplified H<SUB>2</SUB>-producing culture to evaluate its influence on robustness and more particularly on methanogenic activity. An ASBBR was operated over 6 months with unsterile lactose-rich effluent and under Hydraulic Retention Time ranging from 1 to 34 h. Hydrogen performances in terms of productivity and yields ranged from 0.23 to 519 mmole<SUB>H2</SUB> L<SUP>-1</SUP> d<SUP>-1</SUP> and 0.01 to 7.11 mole<SUB>H2</SUB> mole<SUB>lactose</SUB><SUP>-1</SUP>, respectively. No significant methane production was observed all along the experimental procedure, showing that inoculating with a simplified and highly enriched preculture could increase substantially the robustness of the process. Specific preparation of the inoculum may represent a solution to sustain long-term operation of biofilm-based reactors.

2016

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

41

4

pp.2367-2376

10.1016/j.ijhydene.2015.11.131

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74857081

Anaerobic sequencing batch biofilm reactor; Clostridium sp.; Dark fermentation; Pre-enriched consortium