초록
<P><B>Abstract</B></P> <P>Solid state fermentation is a promising technology however rising concerns related to scale up and reproducibility in a productive process. Coffee husk and a specialized inoculum were used in a 4.5L and then in 50L reactors to assess the reproducibility of a cellulase and hemicellulase production system. Fermentations were consistent in terms of cellulase production and microbial communities. The higher temperatures achieved when operating at 50L generated a shift on the microbial communities and a reduction of nearly 50% on cellulase production at pilot scale. In spite, an overall enzymatic production of 3.1±0.5FPUg<SUP>−1</SUP> DM and 48±4Ug<SUP>−1</SUP> DM for FPase and Xyl activities was obtained, respectively, with low deviation coefficients of 16 and 19% for FPase and Xyl production. Gaseous emissions assessment revealed an emission factor of 2.6·10<SUP>−3</SUP> kg volatile organic compounds per Mg of coffee husk and negligible NH<SUB>3</SUB>, CH<SUB>4</SUB> and N<SUB>2</SUB>O emissions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cellulase and xylanase production by SSF from coffee husk was systematically studied. </LI> <LI> A specialized inoculum was used to assess the reproducibility of enzyme production. </LI> <LI> Pilot plant triplicates (50L) revealed a high reproducibility in process evolution. </LI> <LI> Gaseous emissions were low, except for volatile organic compounds. </LI> <LI> A complete microbial characterization of SSF is presented for bacteria and fungi. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>