BioChem - DOS

Biohydrogen production from crude glycerol by two stage of dark and photo fermentation

International journal of hydrogen energy

Chookaew, Teera; O-Thong, Sompong; Prasertsan, Poonsuk

<P><B>Abstract</B></P> <P>Hydrogen production from crude glycerol by two-stage processes of dark fermentation using <I>Klebsiella</I> sp. TR17 and photo fermentation using <I>Rhodopseudomonas palustris</I> TN1 (<I>Rps. palustris</I> TN1) was investigated in batch experiments. In dark fermentation, the cumulative hydrogen production and hydrogen yield was 64.24 mmol H<SUB>2</SUB>/L and 5.74 mmol H<SUB>2</SUB>/g COD consumed, respectively with 80.21% of glycerol conversion rate. The dark fermentation effluent (DFE) was employed for photo fermentation. Effect of DFE concentrations (0&ndash;5 times dilution), with and without supplementation of yeast extract (2.3 g/L) + NaHCO<SUB>3</SUB> (0.63 g/L), and glutamate (2&ndash;8 mM) were optimized. The optimal conditions for hydrogen production from <I>Rps. palustris</I> TN1 were 5 times dilution of DFE without the supplement of yeast extract + NaHCO<SUB>3</SUB>, and 2 mM glutamate. Under the optimum conditions, the cumulative hydrogen production of 3.12 mmol H<SUB>2</SUB>/L and hydrogen yield of 0.68 mmol H<SUB>2</SUB>/g COD consumed was obtained. The total hydrogen yield of two-stage processes was estimated to be 6.42 mmol H<SUB>2</SUB>/g COD consumed which was 10.4% of the theoretical yield.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 5 Times dilution of the dark fermentation effluent was suitable for hydrogen production in photo fermentation. </LI> <LI> Supplementation with 2 mM glutamate gave the highest hydrogen production in photo fermentation. </LI> <LI> The total hydrogen yield in the two-stage processes was 6.42 mmol H<SUB>2</SUB>/g COD consumed. </LI> </UL> </P>

2015

Elsevier

0360-3199

1879-3487

40

24

pp.7433-7438

10.1016/j.ijhydene.2015.02.133

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72132981

Biohydrogen; Crude glycerol; Dark fermentation; Photo fermentation