초록
<P><B>Abstract</B></P> <P> <I>Clostridium thermocellum</I> 27405 is a potential consolidated bioprocessing (CBP) strain due to the efficient lignocellulose degradation ability. Here, the spent mushroom substrate (SMS), a lignocellulosic byproduct of agriculture, was used as a substrate for hydrogen production by <I>C. thermocellum</I>. SMS showed a good performance for hydrogen production and the hydrogen production was significantly enhanced by <I>C. thermocellum</I> supplemented with β-glucosidases, with the highest production reaching 56.0 mM, an increase of 37%. Additionally, β-glucosidase and Triton X-100 had a synergistic effect on biological saccharification, resulted in an accumulation of 5.06 g/L reducing sugars, an increase of 28%. However, fusion β-glucosidase showed no improvement on hydrogen production and biological saccharification. The present study demonstrates the different influences of β-glucosidase and fusion β-glucosidase on hydrogen production and biological saccharification in CBP system and provides valuable information on the biodegradation of SMS using <I>C. thermocellum</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> β-glucosidase significantly enhanced hydrogen production. </LI> <LI> β-glucosidase and Triton X-100 had a synergistic effect on biosaccharification. </LI> <LI> β-glucosidase and Triton X-100 provided a balanced way for bio-refinery. </LI> <LI> Fusion β-glucosidase showed no beneficial effect on the biodegradation of SMS. </LI> </UL> </P>