초록
Hydrogen production from sugarcane bagasse (SCB) by integrating dark-fermentation by Enterobacter aerogenes MTCC 2822 and photo-fermentation by Rhodopseudomonas BHU 01 was investigated. The SCB was hydrolysed by sulphuric acid and the hydrolysate detoxified by passing through adsorbent resin column (Amberlite XAD-4) to remove the inhibitory furfural, and subjected to dark-fermentation. The cellulosic residue from acid hydrolysis was hydrolysed by the new isolate Cellulomonas fimi to release sugars for H<SUB>2</SUB> production by E. aerogenes, through simultaneous saccharification, filtration and fermentation (SSFF). Cumulative H<SUB>2</SUB> production during dark-fermentation and SSFF was 1000 and 613ml/L, respectively. The spent media of dark-fermentation and SSFF were utilized for photo-fermentation by Rhodopseudomonas BHU 01. The cumulative H<SUB>2</SUB> production was 755ml/L for dark-fermentation and 351ml/L for SSFF spent medium.