Evaluation of Mucor indicus and Saccharomyces cerevisiae capability to ferment hydrolysates of rape straw and Miscanthus giganteus as affected by the pretreatment method
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Evaluation of Mucor indicus and Saccharomyces cerevisiae capability to ferment hydrolysates of rape straw and Miscanthus giganteus as affected by the pretreatment method
<P><B>Abstract</B></P> <P>Rape straw and <I>Miscanthus giganteus</I> was pretreated chemically with oxalic acid or sodium hydroxide. The pretreated substrates were hydrolyzed with enzymatic preparations of cellulase, xylanase and cellobiase. The highest concentration of reducing sugars was achieved after hydrolysis of <I>M. giganteus</I> pretreated with NaOH (51.53gdm<SUP>−3</SUP>). In turn, the highest yield of enzymatic hydrolysis determined based on polysaccharides content in the pretreated substrates was obtained in the experiments with <I>M. giganteus</I> and oxalic acid (99.3%). Rape straw and <I>M. giganteus</I> hydrolysates were fermented using yeast <I>Saccharomyces cerevisiae</I> 7, NRRL 978 or filamentous fungus <I>Mucor rouxii</I> (<I>Mucor indicus</I>) DSM 1191. The highest ethanol concentration was determined after fermentation of <I>M. giganteus</I> hydrolysate pretreated with NaOH using <I>S. cerevisiae</I> (1.92% v/v). Considering cellulose content in the pretreated solid, the highest degree of its conversion to ethanol (86.2%) was achieved after fermentation of the hydrolysate of acid-treated <I>M. giganteus</I> using <I>S. cerevisiae</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The comparison of two pretreatment methods of rape straw and <I>M. giganteus</I>. </LI> <LI> The acid treatment results in a significant removal of hemicellulose from the solid. </LI> <LI> The highest reducing sugars concentration was found following alkali pretreatment. </LI> <LI> The used microorganisms revealed a favorable capability for the ethanol fermentation. </LI> </UL> </P>