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Characterization of a new zeaxanthin producing strain of Chlorella saccharophila isolated from New Zealand marine waters

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      2. 계면활성제⁄증점제
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논문

Characterization of a new zeaxanthin producing strain of Chlorella saccharophila isolated from New Zealand marine waters

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Singh, D.; Puri, M.; Wilkens, S.; Mathur, A.S.; Tuli, D.K.; Barrow, C.J.

초록

A fast growing strain of Chlorella saccharophila was isolated from the marine water of New Zealand and grown in heterotrophic conditions using glucose or glycerol as a carbon source. Biomass production was found to be higher in culture fed with glucose (2.14+/-0.08gL<SUP>-1</SUP>) as compared to glycerol (0.378+/-0.04gL<SUP>-1</SUP>). Lipid accumulation was similar for both carbon sources, at approximately 22% of dry cell weight. However, carotenoid yield was higher with glycerol (0.406+/-0.0125mgg<SUP>-1</SUP>) than with glucose (0.21+/-0.034mgg<SUP>-1</SUP>). Further optimization of the growth of the isolate gave maximal carotenoid production of 16.39+/-1.19mgg<SUP>-1</SUP> total carotenoid, containing 11.32+/-0.64mgg<SUP>-1</SUP> zeaxanthin and 5.07+/-0.55mgg<SUP>-1</SUP> &beta;-carotene. Comparison of various chemical and physical carotenoid extraction methods showed that ultrasonication was required for maximum extraction yields. The new strain has potential for biofuel, with carotenoid co-production.

발행연도

2013

발행기관

Elsevier Applied Science

ISSN

0960-8524

143

페이지

pp.308-314

주제어

Carotenoids; RP-HPLC; Ultrasonication; Biofuel; Microalgae

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논문; 2013-09-01

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