초록
A fast growing strain of Chlorella saccharophila was isolated from the marine water of New Zealand and grown in heterotrophic conditions using glucose or glycerol as a carbon source. Biomass production was found to be higher in culture fed with glucose (2.14+/-0.08gL<SUP>-1</SUP>) as compared to glycerol (0.378+/-0.04gL<SUP>-1</SUP>). Lipid accumulation was similar for both carbon sources, at approximately 22% of dry cell weight. However, carotenoid yield was higher with glycerol (0.406+/-0.0125mgg<SUP>-1</SUP>) than with glucose (0.21+/-0.034mgg<SUP>-1</SUP>). Further optimization of the growth of the isolate gave maximal carotenoid production of 16.39+/-1.19mgg<SUP>-1</SUP> total carotenoid, containing 11.32+/-0.64mgg<SUP>-1</SUP> zeaxanthin and 5.07+/-0.55mgg<SUP>-1</SUP> β-carotene. Comparison of various chemical and physical carotenoid extraction methods showed that ultrasonication was required for maximum extraction yields. The new strain has potential for biofuel, with carotenoid co-production.