초록
<P><B>Abstract</B></P> <P>Co-culture of <I>Clostridium acetobutylicum</I> and <I>Saccharomyces cerevisiae</I> was used to enhance solvents (acetone-butanol-ethanol, ABE) production from cassava without amylolytic enzymes addition. Under the simultaneous inoculation condition of <I>C. acetobutylicum</I> and <I>S. cerevisiae</I> with the inoculation level of 5% (v/v) for both strains, 21.74g/L solvents was produced from 75g/L cassava with the yield of 0.36g solvents/g glucose. The solvents titer and yield obtained in the co-culture process increased by 14.3% and 9.1%, respectively, compared to those achieved in ABE fermentation. For further improvements in total solvents titer and yield from cassava, ABE fermentation and co-culture process from higher cassava concentration media were conducted. 42.56g/L total solvents was achieved from 150g/L cassava by co-culture with the yield of 0.46g solvents/g glucose. The solvents titer and yield obtained in the co-culture process increased by 141.0% and 43.8%, respectively, compared to those obtained from ABE fermentation. The results showed great potential for the use of co-culture of <I>C. acetobutylicum</I> and <I>S. cerevisiae</I> for solvents production from starchy substrate.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cassava was used for solvents fermentation without amylolytic enzymes addition. </LI> <LI> Co-culture of <I>Clostridium acetobutylicum</I> and <I>Saccharomyces cerevisiae</I> was conducted. </LI> <LI> Effect of different inoculation methods on solvents titer and yield was evaluated. </LI> <LI> 42.56g/L ABE was achieved from 150g/L cassava by co-culture process. </LI> </UL> </P>