초록
<P><B>Abstract</B></P> <P>The impact of strain selection and culture conditions on bacterial nanocellulose (BNC) productivity and quality was investigated by using four strains, static and agitated cultures, and an initial pH in the range 4-6. With agitation, strain DHU-ATCC-1 displayed highest productivity [1.14 g/(L × d)]. In static cultures, DHU-ZGD-1186 exhibited superior BNC yield on consumed glucose (0.79 g/g), and lowest by-product formation with respect to gluconic acids [≤0.07 g/(L × d)]. By-product formation typically decreased in the order gluconic acid >2-keto-gluconic acid >5-keto-gluconic acid, and was lowest in cultures with high initial pH. The BNC from DHU-ZGD-1186 exhibited higher average viscometric degree of polymerization (DP<SUB>v</SUB>), higher crystallinity index, and higher tear index. In conclusion, both strain selection and cultivation conditions had an impact on BNC productivity and properties. Productivity, DP<SUB>v</SUB>, crystallinity, and mechanical strength of BNC from agitated cultures could be similar to or even higher than the corresponding values for static cultures.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Strain selection and cultivation conditions affected BNC productivity and quality. </LI> <LI> BNC with high productivity and high quality can be provided using agitated cultures. </LI> <LI> Bacteria with low gluconic acids yields would provide high BNC yield. </LI> <LI> One strain produced BNC with a DP<SUB>v</SUB> ≥7800, compared to <5000 for other strains. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>