BioChem - DOS

High efficiency cell-recycle continuous sodium gluconate production by Aspergillus niger using on-line physiological parameters association analysis to regulate feed rate rationally

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Lu, F.; Li, C.; Wang, Z.; Zhao, W.; Chu, J.; Zhuang, Y.; Zhang, S.

In this paper, a system of cell-recycle continuous fermentation for sodium gluconate (SG) production by Aspergillus niger (A. niger) was established. Based on initial continuous fermentation result (100.0h) with constant feed rate, an automatic feedback strategy to regulate feed rate using on-line physiological parameters (OUR and DO) was proposed and applied successfully for the first time in the improved continuous fermentation (240.5h). Due to less auxiliary time, highest SG production rate (31.05+/-0.29gL<SUP>-1</SUP>h<SUP>-1</SUP>) and highest yield (0.984+/-0.067molmol<SUP>-1</SUP>), overall SG production capacity (975.8+/-5.8gh<SUP>-1</SUP>) in 50-L fermentor of improved continuous fermentation increased more than 300.0% compared to that of batch fermentation. Improvement of mass transfer and dispersed mycelia morphology were the two major reasons responsible for the high SG production rate. This system had been successfully applied to industrial fermentation and SG production was greatly improved.

2016

Elsevier Applied Science

0960-8524

220

pp.433-441

10.1016/j.biortech.2016.08.062

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART76461166

Continuous fermentation; Sodium gluconate; Aspergillus niger; Feedback control; On-line parameters