BioChem - DOS

Simultaneous production of citric acid and invertase by Yarrowia lipolytica SUC + transformants

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Lazar, Z.; Walczak, E.; Robak, M.

Simultaneous production of citric acid (CA) and invertase by Yarrowia lipolytica A-101-B56-5 (SUC<SUP>+</SUP> clone) growing from sucrose, mixture of glucose and fructose, glucose or glycerol was investigated. Among the tested substrates the highest concentration of CA was reached from glycerol (57.15g/L) with high yield (Y<SUB>CA/S</SUB>=0.6g/g). When sucrose was used, comparable amount of CA was secreted (45g/L) with slightly higher yield (Y<SUB>CA/S</SUB>=0.643g/g). In all cultures amount of isocitrate (ICA) was below 2% of total citrates. Considering invertase production, the best carbon source appeared to be sucrose (72380U/L). The highest yield of CA and invertase biosynthesis calculated for 1g of biomass was obtained for cells growing from glycerol (9.9g/g and 4325U/g, respectively). Concentrates of extra- and intracellular invertase of the highest activity were obtained from sucrose as substrate (0.5 and 1.8x10<SUP>6</SUP>U/L, respectively).

2011

Elsevier Applied Science

0960-8524

102

13

pp.6982-6989

10.1016/j.biortech.2011.04.032

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56504507

[abr] YII/X; intracellular invertase yield from biomass; Citric acid; Invertase; Yarrowia lipolytica; Sucrose; Glycerol; [abr] CA; citric acid; [abr] ICA; isocitric acid; [abr] EI; extracellular invertase activity; [abr] II; intracellular invertase activity; [abr] QCA; citric acid volumetric productivity; [abr] qCA; specific rate of citric acid production; [abr] QEI; extracellular invertase volumetric productivity; [abr] qEI; specific rate of extracellular invertase production; [abr] QII; intracellular invertase volumetric productivity; [abr] qII; specific rate of intracellular invertase production; [abr] X; biomass; [abr] YCA/S; citric acid yield from substrate; [abr] YCA/X; citric acid yield from biomass; [abr] YEI/S; extracellular invertase yield from substrate; [abr] YEI/X; extracellular invertase yield from biomass; [abr] YII/S; i;