초록
<P>Debaryomyces hansenii NRRL Y-7426 metabolised ferulic acid into different phenolic compounds using a factorial design where glucose concentration (in the range of 1-20?g/L), peptone concentration (2-20?g/L) and yeast extract concentration (0.2-10?g/L) were the independent variables. The interrelationship between dependent and operational variables was well fitted (R (2)?>?0.95) to models including linear, interaction and quadratic terms. Depending on the glucose and nitrogen concentrations, which redirected the metabolism, the major degradation products were 1,226.2?mg 4-vinyl guaiacol/L after 72?h (molar yield of 86.0?%), 1,077.8?mg vanillic acid/L after 360?h (molar yield of 91.1?%) or 1,682.6?mg acetovanillone/L after 408?h (molar yield of 98.8?%) in fermentations carried out with 2,000?mg ferulic acid/L. Other metabolites such as vanillin, vanillyl alcohol or 4-ethylguaiacol were present in lower amounts.</P>