초록
<P><B>Abstract</B></P> <P>The enhanced production of 2,3-butanediol was investigated using a metabolic engineering approach and optimized fermentation conditions. New engineered strains of <I>Enterobacter aerogenes</I> ATCC 29007 were developed by deleting the <SMALL>D</SMALL>-<I>lactate dehydrogenase</I> (<I>ldh</I>A), <I>phosphate acetyltransferase</I> (<I>pta</I>), <I>malate dehydrogenase</I> (<I>mdh</I>), and <I>acetaldehyde dehydrogenase</I> (<I>acdh</I>) genes to block the production of lactate, acetate, succinate, and ethanol, respectively. The resulting engineered strain <I>E. aerogenes</I> SUMI02 (Δ<I>ldh</I>AΔ<I>pta</I>) produced 36.5 g/L of 2,3-butanediol in flask cultivation, an amount 8.11 times greater than that of its wild type counterpart (4.5 g/L). In addition, the 2,3-butanediol production and productivity reached 38.24 g/L and 0.8 g/L/h, respectively, in the batch fermentation using a bioreactor.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Pta, ladhA, mdh and acdh</I> genes were successfully deleted in <I>E. aerogenes</I> ATCC 29007. </LI> <LI> Glucose was used as a carbon source by <I>E. aerogenes</I> to produce 2, 3-butanediol. </LI> <LI> <I>E. aerogenes</I> SUMI02 produced two fold higher 2, 3-butanediol than wild strain. </LI> <LI> Deletion of <I>pta</I> gene increased lactate production but reduced other metabolites. </LI> <LI> By deletion of <I>ldh</I>A and <I>pta</I> genes, 2, 3-butanediol production was maximized. </LI> </UL> </P>