BioChem - DOS

Effects of organic solvents on activity and conformation of recombinant Candida antarctica lipase A produced by Pichia pastoris

Process biochemistry

Yang, C.; Wang, F.; Lan, D.; Whiteley, C.; Yang, B.; Wang, Y.

The effect of various organic solvents on the hydrolytic and esterification activities of lipase A from Candida antarctica (CALA) was investigated. Compared to the control group, the esterification and hydrolytic activities of CALA both increased with treatment of acetonitrile or acetone. However, the catalytic activity decreased after treatment with other organic solvents, especially with ethanol or ethyl acetate. Moreover, with treatment by the same organic solvents, the residual esterification activity of CALA was much higher than the hydrolytic activity for the olive oil. Results of Fourier transform-infrared spectroscopy analysis implied that the catalytic activity variance was mainly attributed to the secondary structure changes of CALA. The acetonitrile treatment could also increase the thermal and pH stability of CALA.

2012

Elsevier Applied Science

1359-5113

47

3

pp.533-537

10.1016/j.procbio.2011.11.017

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58353556

Candida antarctica lipase A; Organic solvents; Catalytic activity; Secondary structure; Fourier transform-infrared spectroscopy