초록
<P><B>Abstract</B></P> <P>The present study aimed to explore a novel attribute of the bacterium <I>Lactobacillus hilgardii</I> IITRKH159, isolated from Sapodilla, which was found to produce Bacterial cellulose. The compositional analysis of BC was carried out by HPLC system using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone showed that it consisted of glucan. The Physiochemical study of BC showed that it had bacterial nano-cellulose like structure, immensely pure, highly crystalline (90.25%) and significantly enriched with I<SUB>α</SUB> form of cellulose. Additionally, FE-SEM and TEM of the BC revealed the morphological similarities with bacterial nano-cellulose due to the presence of a reticulated structure consisting of nano-sized fibrils with an average width of 45 nm. The effect of different carbon sources was analyzed to evaluate BC production and demonstrated the production of 7.23 ± 0.59 g L<SUP>−1</SUP> BC with conversions yield (0.30 g BC/g sugar) and productivity (0.45 ± 0.15 g L<SUP>−1</SUP> d<SUP>−1</SUP>) in a modified Y-medium after 16 days of cultivation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Lactobacillus hilgardii</I> is herein shown to be a novel bacterium capable of producing BC. </LI> <LI> The composition of BC was analyzed by acid hydrolysis followed by pre-column derivatization HPLC. </LI> <LI> The BC film was characterized by ATR-FTIR, XRD, FE-SEM, TEM, <SUP>13</SUP>C CP/ MAS NMR, and TG/DTG. </LI> <LI> The new strain showed the high productivity of BC in fructose-containing Y medium. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>