초록
<P><B>Background</B></P><P>The white-rot fungus <I>Phlebia</I> sp. MG-60 shows valuable properties such as high ethanol yield from several lignocellulosic materials, although white-rot fungi commonly degrade woody components to CO<SUB>2</SUB> and H<SUB>2</SUB>O. In order to identify genes involved in ethanol production by <I>Phlebia</I> sp. MG-60, we compared genes differentially expressed by the ethanol producing fungus <I>Phlebia</I> sp. MG-60 and the model white-rot fungus <I>Phanerochaete chrysosporium</I> under ethanol fermenting and non-fermenting conditions using next-generation sequencing technologies.</P><P><B>Results</B></P><P>mRNAs from mycelia of <I>Phlebia</I> sp. MG-60 and <I>P. chrysosporium</I> under fermenting and non-fermenting conditions were sequenced using the MiSeq system. To detect differentially expressed genes, expression levels were measured in fragments per kilobase of exon per million mapped reads (FPKM). Differentially expressed genes were annotated using BLAST searches, Gene Ontology classifications, and KEGG pathway analysis. Functional analyses of differentially expressed genes revealed that genes involved in glucose uptake, glycolysis, and ethanol synthesis were widely upregulated in <I>Phlebia</I> sp. MG-60 under fermenting conditions.</P><P><B>Conclusions</B></P><P>In this study, we provided novel transcriptomic information on <I>Phlebia</I> sp. MG-60, and these RNA-seq data were useful in targeting genes involved in ethanol production for future genetic engineering.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s12864-016-2977-7) contains supplementary material, which is available to authorized users.</P>