Tween 80 influences the production of intracellular lipase by Schizochytrium S31 in a stirred tank reactor

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바이오화학분류

- 바이오정밀화학
  1. 용매
  2. 화학제품
  3. 기타
- 화장품용 기능성소재
  1. 계면활성제⁄증점제
- 의료용 화학소재
  1. 치료제
  2. 건강보조식품
  3. 식품첨가제

논문

Tween 80 influences the production of intracellular lipase by Schizochytrium S31 in a stirred tank reactor

학술지

Process biochemistry

저자명

Byreddy, A.R.; Rao, N.M.; Barrow, C.J.; Puri, M.

초록

Marine microorganisms are a potential source of enzymes with structural stability, high activity at low temperature and unique substrate selectivity. Thraustochytrids are marine heterotrophic microbes, well known for the production of omega-3 fatty acids. In this study the effect of Tween 80 as a carbon source was investigated with regard to biomass, lipase and lipid productivity in Schizochytrium sp. S31. Tween 80 (1%) and 120h of incubation were the optimum condition period for biomass, lipid and lipase productivity in a stirred tank reactor. The yields obtained were 0.9gL<SUP>-1</SUP> of biomass, 300mgg<SUP>-1</SUP> of lipid and 39U/g of lipase activity. Sonication was optimised in terms of time and acoustic power to maximise the yield of extracted lipase. The extracted lipase from Schizochytrium S31 was observed to hydrolyse long chain polyunsaturated fatty acids DHA and EPA.

발행연도

2017

발행기관

Elsevier Applied Science

ISSN

1359-5113

권

페이지

pp.30-35

DOI

10.1016/j.procbio.2016.11.026

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