BioChem - DOS

Enzymatic saccharification of brown seaweed for production of fermentable sugars

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Sharma, Sandeep; Horn, Svein Jarle

<P><B>Abstract</B></P> <P>This study shows that high drying temperatures negatively affect the enzymatic saccharification yield of the brown seaweed <I>Saccharina latissima</I>. The optimal drying temperature of the seaweed in terms of enzymatic sugar release was found to be 30&deg;C. The enzymatic saccharification process was optimized by investigating factors such as kinetics of sugar release, enzyme dose, solid loading and different blend ratios of cellulases and an alginate lyase. It was found that the seaweed biomass could be efficiently hydrolysed to fermentable sugars using a commercial cellulase cocktail. The inclusion of a mono-component alginate lyase was shown to improve the performance of the enzyme blend, in particular at high solid loadings. At 25% dry matter loading a combined glucose and mannitol concentration of 74g/L was achieved.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Saccharina latissima</I> can be efficiently hydrolysed to fermentable sugars. </LI> <LI> High drying temperatures negatively affect enzymatic saccharification yield. </LI> <LI> The efficiency of a cellulase cocktail was improved by inclusion of alginate lyase. </LI> <LI> At 25% solid loading a sugar concentration of 74g/L was achieved. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2016

Elsevier

0960-8524

213

pp.155-161

10.1016/j.biortech.2016.02.090

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75763535

Mannitol; Laminarin; Biorefining; Macroalgae; Kelp