초록
<P>Sustained H<SUB>2</SUB> photoproduction is demonstrated in green algae under a train of strong white light pulses interrupted by longer dark phases. The devised protocol relies on the presence of the [FeFe]-hydrogenase in algal chloroplasts, which is activated within a few seconds after the establishment of anaerobiosis. H<SUB>2</SUB> photoproduction proceeds for up to 3 days with the maximum rate occurring in the first 6 hours.</P><P>Graphic Abstract</P><P>Pulse-illumination re-directs photosynthetic electron flow destined for CO<SUB>2</SUB> fixation to [FeFe]-hydrogenase, thus sustaining algal H<SUB>2</SUB> photoproduction for 70 hours.<BR/><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c8ee00054a'/><BR/></P>