초록
<P><B>Abstract</B></P> <P>Reducing the lignin inhibition in the enzymatic digestibility is essential to obtain high yields of sugar and bioethanol. The sequential NaOH and hydroxymethylation pretreatment (AHP) were used to treat sugarcane bagasse (SCB). Compared with NaOH pretreated SCB alone (AP-SCB), AHP increased the yield of glucose and xylose from 53.3%–68.88 %, from 67.8%–74.7 %, respectively. The hydrolysate was fermented using Saccharomyces cerevisiae for 24 h, and then ethanol concentration was 10.67 g/L, which increased by approximately 13 % compared with that of AP-SCB. The post-treatment of hydroxymethylation introduced hydroxymethyl groups and hydroxylic groups into in the aromatic nucleus of lignin, increasing the hydrophilicity and solubility of lignin in materials, which can reduce the nonproductive adsorption of lignin on cellulases and thereby improve the enzymatic saccharification efficiency.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hydroxymethylation post-treatment reduced lignin inhibition on enzymatic hydrolysis. </LI> <LI> Glucose yield of AHP-SCB was 68.33 %, increased by 27 % than that of AP-SCB. </LI> <LI> Ethanol concentration of AHP-SCB was 10.67 g/L, increased by 13 % than that of AP-SCB. </LI> <LI> Hydroxymethylation introduced hydrophilic groups into aromatic nucleus of lignin. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Synopsis: The effect of sequential NaOH and hydroxymethylation pretreatment (AHP) on enzymatic hydrolysis and fermentation are being investigated for obtaining high yields of glucose and ethanol.</P> <P>[DISPLAY OMISSION]</P>