Production of succinate and polyhydroxyalkanoate from substrate mixture by metabolically engineered Escherichia coli

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바이오화학분류

- 바이오플라스틱
  1. 고무
  2. 플라스틱
- 바이오정밀화학
  1. 용매
  2. 화학제품
- 화장품용 기능성소재
  1. 계면활성제⁄증점제
- 의료용 화학소재
  1. 식품첨가제

논문

Production of succinate and polyhydroxyalkanoate from substrate mixture by metabolically engineered Escherichia coli

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Kang, Z.; Du, L.; Kang, J.; Wang, Y.; Wang, Q.; Liang, Q.; Qi, Q.

초록

The strategic design of this study aimed at producing succinate and polyhydroxyalkanoate (PHA) from substrate mixture of glycerol/glucose and fatty acid in Escherichia coli. To accomplish this, an E. coli KNSP1 strain derived from E. coli LR1110 was constructed by deletions of ptsG, sdhA and pta genes and overexpression of phaC1 from Pseudomonas aeruginosa. Cultivation of E. coli KNSP1 showed that this strain was able to produce 21.07g/L succinate and 0.54g/L PHA (5.62wt.% of cell dry weight) from glycerol and fatty acid mixture. The generated PHA composed of 58.7mol% 3-hydroxyoctanoate (3HO) and 41.3mol% 3-hydroxydecanoate (3HD). This strain would be useful for complete utilization of byproducts glycerol and fatty acid of biodiesel production process.

발행연도

2011

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

102

호

페이지

pp.6600-6604

DOI