BioChem - DOS

Downstream separation and purification of succinic acid from fermentation broths using spent sulphite liquor as feedstock

Separation and purification technology

Alexandri, Maria; Vlysidis, Anestis; Papapostolou, Harris; Tverezovskaya, Olga; Tverezovskiy, Viacheslav; Kookos, Ioannis K.; Koutinas, Apostolis

<P><B>Abstract</B></P> <P>Spent sulphite liquor produced as side stream from sulphite pulping of <I>Eucalyptus globulus</I> hardwood could be used for the separation of lignosulphonates by nanofiltration in the retentate stream and succinic acid production via fermentation of the permeate stream by <I>Actinobacillus succinogenes</I> or <I>Basfia succiniciproducens</I>. The potential integration of this process in conventional pulp mills towards the development of a novel biorefinery is dependent on the efficient downstream separation of succinic acid crystals at high yield and purity. This study focuses on the evaluation of five downstream separation processes, namely calcium precipitation, direct crystallisation using acidification or cation-exchange resins, salting-out and reactive extraction, for the purification of succinic acid from crude fermentation broths. Reactive extraction using trioctylamine in 1-hexanol and direct crystallisation coupled with cation-exchange resins led to succinic acid recovery yields of 73% and 79%, respectively. <SUP>1</SUP>H NMR analysis showed that these downstream separation processes led to succinic acid crystal purities of <I>ca</I> 98.5% for reactive extraction and higher than 99% for the direct crystallisation method coupled with cation-exchange resins with no detectable acetic acid content when re-crystallisation was employed. It has been demonstrated that succinic acid produced via fermentation using side streams from pulp and paper mills could be separated at high purity and yield from crude fermentation broths rendering feasible its utilisation for poly(butylene succinate) production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Purification of succinic acid from SSL-based fermentation media has been evaluated. </LI> <LI> Acidification with cation-exchange resins resulted in high succinic acid purity. </LI> <LI> Re-crystallisation of the succinic acid crystals led to a purity of more than 99%. </LI> </UL> </P>

2019

Elsevier

1383-5866

209

pp.666-675

10.1016/j.seppur.2018.08.061

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART91099516

https://research.bangor.ac.uk/portal/files/21802223/Downstream_separation_and_purification_of_succinic_acid_from_fermentation_broths_using_spent_sulphite_liquor_as_feedstock.pdf

Succinic acid; Spent sulphite liquor; Downstream separation process; Basfia succiniciproducens; Actinobacillus succinogenes