BioChem - DOS

Influence of linoleic acid, pH and HRT on anaerobic microbial populations and metabolic shifts in ASBRs during dark hydrogen fermentation of lignocellulosic sugars

International journal of hydrogen energy

Chaganti, S.R.; Pendyala, B.; Lalman, J.A.; Veeravalli, S.S.; Heath, D.D.

In this study, controlling an anaerobic microbial community to increase the hydrogen (H<SUB>2</SUB>) yield during the degradation of lignocelluosic sugars was accomplished by adding linoleic acid (LA) at low pH and reducing the hydraulic retention time (HRT) of an anaerobic sequencing batch reactor (ASBR). At pH 5.5 and a 1.7 d HRT, the maximum H<SUB>2</SUB> yield for LA treated cultures fed glucose or xylose reached 2.89 +/- 0.18 mol mol<SUP>-1</SUP> and 1.94 +/- 0.17 mol mol<SUP>-1</SUP>, respectively. The major soluble metabolites at pH 5.5 with a 1.7 day HRT differed between the control and LA treated cultures. A metabolic shift toward H<SUB>2</SUB> production resulted in increased hydrogenase activity in both the xylose (13%) and glucose (34%) fed LA treated cultures relative to the controls. In addition, the Clostridia population and the H<SUB>2</SUB> yield were elevated in cultures treated with LA. A flux balance analysis for the LA treated cultures showed a reduction in homoacetogenic activity which was associated with reducing the Bacteriodes levels from 12% to 5% in the glucose fed cultures and 16% to 10% in the xylose fed cultures. Strategies for controlling the homoacetogens and optimal hydrogen production from glucose and xylose are proposed.

2013

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

38

5

pp.2212-2220

10.1016/j.ijhydene.2012.11.137

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65418260

Hydrogen; Glucose; Xylose; Metabolic flux analysis; Microbial diversity; Anaerobic sequencing batch reactor