초록
<P><B>Abstract</B></P> <P> <I>Corynebacterium glutamicum</I> is used for production of the food and feed amino acids <SMALL>L</SMALL>-glutamate and <SMALL>L</SMALL>-lysine at the million-ton-scale. One feed formulation of <SMALL>L</SMALL>-lysine simply involves spray-drying of the fermentation broth, thus, including secreted <SMALL>L</SMALL>-lysine and <I>C. glutamicum</I> cells which are pigmented by the C50 carotenoid decaprenoxanthin. <I>C. glutamicum</I> has been engineered for overproduction of various compounds including carotenoids. In this study, <I>C. glutamicum</I> was engineered for coproduction of a secreted amino acid with a cell-bound carotenoid. Asa proof of principle, coproduction of <SMALL>L</SMALL>-glutamate with the industrially relevant astaxanthin was shown. This strategy was applied to engineer <SMALL>L</SMALL>-lysine overproducing strains for combined overproduction of secreted <SMALL>L</SMALL>-lysine with the cell-bound carotenoids decaprenoxanthin, lycopene, β-carotene, zeaxanthin, canthaxanthin and astaxanthin. By fed-batch fermentation 48g/L <SMALL>L</SMALL>-lysine and 10mg/L astaxanthin were coproduced. Moreover, <I>C. glutamicum</I> was engineered for coproduction of <SMALL>L</SMALL>-lysine and β-carotene from xylose and arabinose as alternative feedstocks.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Coproduction of a cell-bound and a secreted product by engineered <I>C. glutamicum.</I> </LI> <LI> Fermentative coproduction of carotenoids with <SMALL>L</SMALL>-glutamate or <SMALL>L</SMALL>-lysine established. </LI> <LI> Alternative feedstocks as basis for coproduction of <SMALL>L</SMALL>-lysine and β-carotene. </LI> <LI> Astaxanthin and <SMALL>L</SMALL>-lysine produced as two feed supplements from one fermentation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>