BioChem - DOS

Optimization of Isomaltooligosaccharide Size Distribution by Acceptor Reaction of Weissella confusa Dextransucrase and Characterization of Novel α-(1→2)-Branched Isomaltooligosaccharides

Journal of agricultural and food chemistry

Shi, Qiao; Hou, Yaxi; Juvonen, Minna; Tuomainen, Pä ivi; Kajala, Ilkka; Shukla, Shraddha; Goyal, Arun; Maaheimo, Hannu; Katina, Kati; Tenkanen, Maija

<P>Long-chain isomaltooligosaccharides (IMOs) are promising prebiotics. IMOs were produced by a <I>Weissella confusa</I> dextransucrase via maltose acceptor reaction. The inputs of substrates (i.e., sucrose and maltose, 0.15&ndash;1 M) and dextransucrase (1&ndash;10 U/g sucrose) were used to control IMO yield and profile. According to response surface modeling, 1 M sucrose and 0.5 M maltose were optimal for the synthesis of longer IMOs, whereas the dextransucrase dosage showed no significant effect. In addition to the principal linear IMOs, a homologous series of minor IMOs were also produced from maltose. As identified by MS<SUP><I>n</I></SUP> and NMR spectroscopy, the minor trisaccharide contained an &alpha;-(1&rarr;2)-linked glucosyl residue on the reducing residue of maltose and thus was &alpha;-<SMALL>d</SMALL>-glucopyranosyl-(1&rarr;2)-[&alpha;-<SMALL>d</SMALL>-glucopyranosyl-(1&rarr;4)]-<SMALL>d</SMALL>-glucopyranose (centose). The higher members of the series were probably formed by the attachment of a single unit branch to linear IMOs. This is the first report of such &alpha;-(1&rarr;2)-branched IMOs produced from maltose by a dextransucrase.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2016/jafcau.2016.64.issue-16/acs.jafc.6b01356/production/images/medium/jf-2016-013568_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf6b01356'>ACS Electronic Supporting Info</A></P>

2016

American Chemical Society

0021-8561

1520-5118

64

16

pp.3276-3286

10.1021/acs.jafc.6b01356

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75360667

isomaltooligosaccharides; dextransucrase; maltose acceptor reaction; response surface modeling; α-(1→2)-linkage; centose