초록
<P><B>Abstract</B></P><P><I>The aim of this work was to maximize the production and the partial characterization of carboxymethylcellulase (CMCase) and filter paper enzyme activity (FPase) using a new isolate by solid state fermentation with agro-industrial substrates and potential application of hydrolytic cellulosic substrates. Four isolates were initially evaluated, and the isolate I-130, identified as</I> Penicillium <I>sp., was selected for FPase and CMCase production. The maximized conditions for the enzyme bioproduction were inoculation of 5 to 7 log spores/g, in soybean hull, at 70% moisture for 7 d at 30°C. The best solvent for extracting the enzymes was citrate buffer (0.05 M, pH 4.5) with a time of 30 min, temperature of 50°C, and agitation of 195.5 and 125 rpm for CMCase and FPase, respectively. The optimum pH and temperature for FPase and CMCase activity were 4.9 and 50°C. Both enzymes were more stable at pH 4.9. FPase and CMCase showed thermal stability only up to 40°C, with rapid deactivation at higher temperatures; the FPase being more stable at this temperature range. The deactivation energy was 89.3 and 78.0 KJ/mol/ for FPase and CMCase, respectively. The highest hydrolytic activity occurred at 350 rpm for soybean hulls (22%) and corn stover and cobs (4.4%).</I></P>