BioChem - DOS

Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms

Journal of industrial microbiology & biotechnology

Meng, Xin; Yang, Jianming; Cao, Yujin; Li, Liangzhi; Jiang, Xinglin; Xu, Xin; Liu, Wei; Xian, Mo; Zhang, Yingwei

<P>Unlike many oleaginous microorganisms, E. coli only maintains a small amount of natural lipids in cells, impeding its utility to overproduce fatty acids. In this study, acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus was expressed in E. coli to redirect the carbon flux to the generation of malonyl-CoA, which resulted in a threefold increase in intracellular lipids. Moreover, providing a high level of NADPH by overexpressing malic enzyme and adding malate to the culture medium resulted in a fourfold increase in intracellular lipids (about 197.74 mg/g). Co-expression of ACC and malic enzyme resulted in 284.56 mg/g intracellular lipids, a 5.6-fold increase compared to the wild-type strain. This study provides some attractive strategies for increasing lipid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms, which could aid the development of a prokaryotic fatty acid producer.</P>

2011

Oxford University Press

1367-5435

1476-5535

38

8

pp.919-925

10.1007/s10295-010-0861-z

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56935770