초록
<P><B>Graphical abstract</B></P><P><ce:figure id='fig0005'></ce:figure></P><P><B>Highlights</B></P><P>► <I>Saccharomyces cerevisiae</I> suspension was incubated in a high-pressure batch reactor in SC CO<SUB>2</SUB>. ► Incubation time was changed regarding to the survival of the yeast <I>S. cerevisiae</I>. ► The protein concentration in suspension of <I>S. cerevisiae</I> in PDB and SPB medium after treatment in SC CO<SUB>2</SUB> increased with increase in pressure. ► ADH from <I>S. cerevisiae</I> was obtained in the active form after incubation of cell suspension in SC CO<SUB>2</SUB>. ► Cytoplasmatic materials were extracted from the yeast cells.</P> <P><B>Abstract</B></P><P>In this study, supercritical carbon dioxide (SC CO<SUB>2</SUB>) was used to obtain proteins and enzymes from yeast <I>Saccharomyces cerevisiae</I>.</P><P>The changes in viability and cell morphology, the release of the cellular proteins and the activity alteration of the enzyme alcohol dehydrogenase (ADH) from <I>S. cerevisiae</I> resulting from the exposure to SC CO<SUB>2</SUB> were investigated. Before the treatment of <I>S. cerevisiae</I> in SC CO<SUB>2</SUB>, the number of viable cells was cca. 10<SUP>5</SUP> colony forming units per mL of cell suspension (cfu/mL). The suspension of the <I>S. cerevisiae</I> culture was incubated in SC CO<SUB>2</SUB> at different pressures (7.5, 15 and 30MPa) for different treatment times (30–300min) and at constant temperature (35°C). The influences of these parameters on total protein concentration, ADH activity and changes in absorbance of nucleic acids (NA) in the suspension of <I>S. cerevisiae</I> during SC CO<SUB>2</SUB> treatment were studied. A decrease in number of living cells of <I>S. cerevisiae</I> in potato dextrose broth (PDB) or in sodium pyrophosphate buffer (SPB) exposed to SC CO<SUB>2</SUB> at all studied pressures was determined when the treatment time was increased. The highest activity of ADH after treatment of the yeast culture suspended in PDB in SC CO<SUB>2</SUB> was detected at a pressure of 7.5MPa and at the treatment time of 120min.The use of SC CO<SUB>2</SUB> is a suitable option to achieve cell death and consequently the secretion of proteins and ADH from cells of <I>S. cerevisiae</I>.</P>