BioChem - DOS

Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol using resting cells of the lactate dehydrogenase-deficient recombinant Klebsiella pneumoniae overexpressing an aldehyde dehydrogenase

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Kumar, V.; Sankaranarayanan, M.; Durgapal, M.; Zhou, S.; Ko, Y.; Ashok, S.; Sarkar, R.; Park, S.

In the present study, the lactate dehydrogenase-deficient (ldhA<SUP>-</SUP>) recombinant Klebsiella pneumoniae overexpressing an ALDH (KGSADH) was developed and the co-production of 3-HP and PDO from glycerol by this recombinant under resting cell conditions was examined. The new recombinant did not produce any appreciable lactate, which seriously inhibits the production of 3-HP and PDO. The final titers of 3-HP and PDO by the ldhA<SUP>-</SUP> recombinant strain at 60h were 252.2mM and 308.7mM, respectively, which were improved by approximately 30% and 50%, respectively, compared to those by the counterpart recombinant strain, which was the wild type for ldhA. In addition, after deleting ldhA, the cumulative yield on glycerol and specific production rate of these two metabolites (3-HP and PDO) were enhanced by 41.4% and 52%, respectively.

2013

Elsevier Applied Science

0960-8524

135

pp.555-563

10.1016/j.biortech.2012.11.018

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65836500

K. pneumoniae J2B KGSADH; K. pneumoniae J2B ΔldhA KGSADH; 3-Hydroxypropionic acid; 1,3-Propanediol; Lactate dehydrogenase