초록
<P><B>Abstract</B></P> <P>A process of isobutanol production from sugarcane bagasse hydrolysates in <I>Enterobacter aerogenes</I> was developed here with a pervaporation-integrated procedure. Isobutanol pathway was overexpressed in a mutant strain with eliminated byproduct-forming enzymes (LdhA, BudA, and PflB). A glucose-and-xylose–coconsuming <I>ptsG</I> mutant was constructed for effective utilization of lignocellulosic biomass. Toxic effects of isobutanol were alleviated by <I>in situ</I> recovery via a pervaporation procedure. Compared to single-batch fermentation, cell growth and isobutanol titer were improved by 60% and 100%, respectively, in the pervaporation-integrated fermentation process. A lab-made cross-linked polydimethylsiloxane membrane was cast on polyvinylidene fluoride and used in the pervaporation process. The membrane-penetrating condensate contained 55–226 g m<SUP>−2</SUP> h<SUP>−1</SUP> isobutanol with 6–25 g L<SUP>−1</SUP> ethanol after separation. This study offers improved fermentative production of isobutanol from lignocellulosic biomass with a pervaporation procedure.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>E. aerogenes</I> was engineered for producing isobutanol from lignocellulosic biomass. </LI> <LI> Sugarcane bagasse hydrolysates was applied to isobutanol production. </LI> <LI> A pervaporation procedure was adopted for <I>in situ</I> recovery of isobutanol. </LI> <LI> The pervaporation-coupled fermentation process improved isobutanol production. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>