BioChem - DOS

Cellulosic hydrogen production and microbial community characterization in hyper-thermophilic continuous bioreactor

International journal of hydrogen energy

Gadow, S.I.; Jiang, H.; Hojo, T.; Li, Y.Y.

A continuous stirred tank reactor was used for the dark hydrogen fermentation of cellulose by mixed microflora at hyper-thermophilic temperature (70 +/- 1 <SUP>o</SUP>C) for 240 days. A total of twenty six batch experiments were conducted to investigate the effect of temperature on the activity of cellulosic-hydrogen producing bacteria. The results show that the system reached a steady state condition after 90 days. A stable hydrogen yield of 7.07 +/- 0.23 mmol H<SUB>2</SUB>/g cellulose was maintained for 150 days with acetate, butyrate, ethanol and propionate as main soluble byproducts. Analysis of 16S rRNA sequences showed that the cellulolytic bacteria were close to Thermoanaerobacterium genus. The cellulosic-hydrogen producing bacteria were able to utilize the cellulose or glucose within a wide range of fermentation temperatures (45-80 <SUP>o</SUP>C) to produce hydrogen. The activation energy for cellulose and glucose were estimated at 133.2 and 117.7 kJ/mol, respectively.

2013

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

38

18

pp.7259-7267

10.1016/j.ijhydene.2013.04.041

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART66081433

Dark fermentation; Cellulose; Hyper-thermophilic; Thermoanaerobacterium; Activity test