BioChem - DOS

High cell density cultivation of Lipomyces starkeyi for achieving highly efficient lipid production from sugar under low C/N ratio

Biochemical engineering journal

Amza, Rezky Lastinov; Kahar, Prihardi; Juanssilfero, Ario Betha; Miyamoto, Nao; Otsuka, Hiromi; Kihira, Chie; Ogino, Chiaki; Kondo, Akihiko

<P><B>Abstract</B></P> <P>Oleaginous microorganisms such as oleaginous yeasts are well known for the ability to accumulate intracelular lipid more than 20% w/w in certain conditions. <I>Lipomyces starkeyi</I> D35 (Ls-D35 strain) is one of promising oleaginous yeast for lipid production. The purpose of this research is to establish the optimum condition for cell and lipid production of Ls-D35 strain under low C/N ratio (17.9) by high cell density cultivation. The optimum pH for cell and lipid production was achieved at pH 5 by using batch cultivation. Four different feeding strategies were conducted to observe the cell and lipid production of Ls-D35 strain. The highest cell yield was achieved by mixed glucose and xylose as substrates (0.15 w/w substrate) after 96 h and the highest lipid yield was achieved by single xylose feeding (0.13 w/w substrate) after 120 h. Meanwhile, the two most fatty acids content were C18:1 and C16:0 which are similar to the content of vegetable oil. These results showed that Ls-D35 strain could produce high cell and optimum lipid under low initial C/N ratio medium which can be an alternative for biodiesel production and other industrial products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The fermentation of Ls-D35 was carried out at low initial C/N ratio (17.9). </LI> <LI> The fermentation of Ls-D35 was optimum at pH 5 for cell and lipid productions. </LI> <LI> The highest lipid yield was achieved by using single xylose feeding. </LI> </UL> </P>

2019

Elsevier

elsevier-specific: oa user license

1369-703x

149

pp.107236

10.1016/j.bej.2019.05.013

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART97555284

http://manuscript.elsevier.com/S1369703X19301639/pdf/S1369703X19301639.pdf

Oleaginous yeast; Lipomyces starkeyi D35; High cell density culture; Cell and lipid productions