BioChem - DOS

Transaminases Applied to the Synthesis of High Added-Value Enantiopure Amines

Organic process research & development

Paul, Caroline E.; Rodrí guez-Mata, Marí a; Busto, Eduardo; Lavandera, Ivá n; Gotor-Ferná ndez, Vicente; Gotor, Vicente; Garcí a-Cerrada, Susana; Mendiola, Javier; de Frutos, Ó scar; Collado, Ivá n

<P>Critical parameters affecting the stereoselective amination of (hetero)aromatic ketones using transaminases have been studied, such as temperature, pH, substrate concentration, cosolvent, and source and percentage of amino donor, to further optimize the production of enantiopure amines using both (<I>S</I>)- and (<I>R</I>)-selective biocatalysts from commercial suppliers. Interesting enantiopure amino building blocks have been obtained, overcoming some limitations of traditional chemical synthetic methods. Representative processes were scaled up, affording halogenated and heteroaromatic amines in enantiomerically pure form and good isolated yields.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/oprdfk/2014/oprdfk.2014.18.issue-6/op4003104/production/images/medium/op-2013-003104_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/op4003104'>ACS Electronic Supporting Info</A></P>

2014

American Chemical Society

cc-by-nc-nd

1083-6160

1520-586x

18

6

pp.788-792

10.1021/op4003104

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART69561379

https://digibuo.uniovi.es/dspace/bitstream/10651/32972/1/Organic%20Process%20Research%20and%20Development%2c%2018%286%29%2c%20p.%20788-792%20%282014%29.pdf