초록
<P><B>Abstract</B></P> <P>In this study, a β-glucosidase from <I>Paenibacillus</I> sp. M1 was expressed in <I>E. coli</I> BL21(DE3), purified and characterized. The specific activity of purified BglA was 137.64U·mg<SUP>−1</SUP> protein with optimal temperature and pH of 50°C and 6.0. Furthermore, BglA shows excellent adaption to various environmental factors such as temperature, pH and metal ions. Engineered <I>E. coli</I> Suc260 was further reconstructed by overexpressing the β-glucosidase for achieving direct cellobiose utilization, which could efficiently utilize the pretreated sugarcane bagasses hydrolysate (SBH) consisting of 25.30g·L<SUP>−1</SUP> cellobiose, 9.70g·L<SUP>−1</SUP> glucose, 5.90g·L<SUP>−1</SUP> arabinose and 7.10g·L<SUP>−1</SUP> xylose. As a result, 26.50g·L<SUP>−1</SUP> and 24.30g·L<SUP>−1</SUP> succinic acid were produced by strain Suc260(pTbglA) from cellobiose and SBH with corresponding yields of 88.30% and 89.20% using dual-phase fermentation, respectively. This study indicated that incomplete enzymatic hydrolysate of SCB will be a potential feedstock for succinic acid production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A β-glucosidase from <I>Paenibacillus</I> sp. was cloned. </LI> <LI> The recombinant enzyme BglA was expressed, purified and characterized. </LI> <LI> The engineered <I>E. coli</I> was constructed for succinate production using cellobiose. </LI> <LI> Sugarcane bagasse hydrolysate could be utilized for bio-based chemicals production. </LI> </UL> </P>