초록
Biohydrogen has been regarded as carbon neutral fuel. Thus it possesses tremendous potential in energy sector. The present study deals with the potentiality of bio-augmented facultative anaerobic bacteria with obligate anaerobe for the improvement of H<SUB>2</SUB> production. The co-culture strategy of Klebsellia pneumoniae and Clostridium acetobutylicum improved hydrogen yield by 37% and 18% respectively as compared to individual organism. COD removal efficiency was also observed higher in case of co-culture. Maximum H<SUB>2</SUB> yield by this augmented system using cane molasses, starchy wastewater and distillery effluent were 9.47, 8.72 and 7.78 mol H<SUB>2</SUB> kg<SUP>-1</SUP> COD<SUB>reduced</SUB> respectively. The COD removal efficiency using different organic residues were in the range of 50-70%. Highest H<SUB>2</SUB> production rate of 1125, 642 and 790 mL L<SUP>-1</SUP> h<SUP>-1</SUP> were observed by using cane molasses, starchy wastewater and distillery effluent respectively in CSTR. So, bio-augmented system could be helpful in realizing the goal of ''waste to energy'' concept.