초록
<B>Abstract</B>Background<P>Aromatic amino acids and their derivatives are valuable chemicals and are precursors for different industrially compounds. <I>p</I>-Coumaric acid is the main building block for complex secondary metabolites in commercial demand, such as flavonoids and polyphenols. Industrial scale production of this compound from yeast however remains challenging.</P>Results<P>Using metabolic engineering and a systems biology approach, we developed a <I>Saccharomyces cerevisiae</I> platform strain able to produce 242 mg/L of <I>p</I>-coumaric acid from xylose. The same strain produced only 5.35 mg/L when cultivated with glucose as carbon source. To characterise this platform strain further, transcriptomic analysis was performed, comparing this strain’s growth on xylose and glucose, revealing a strong up-regulation of the glyoxylate pathway alongside increased cell wall biosynthesis and unexpectedly a decrease in aromatic amino acid gene expression when xylose was used as carbon source.</P>Conclusions<P>The resulting <I>S. cerevisiae</I> strain represents a promising platform host for future production of <I>p</I>-coumaric using xylose as a carbon source.</P>