BioChem - DOS

Whole-cell Gluconobacter oxydans biosensor for 2-phenylethanol biooxidation monitoring

Analytica chimica acta : an international journal devoted to all branches of analytical chemistry

Schenkmayerova, A.; Bertokova, A.; Sefcovicova, J.; Stefuca, V.; Bucko, M.; Vikartovska, A.; Gemeiner, P.; Tkac, J.; Katrlik, J.

A microbial biosensor for 2-phenylethanol (2-PE) based on the bacteria Gluconobacter oxydans was developed and applied in monitoring of a biotechnological process. The cells of G. oxydans were immobilized within a disposable polyelectrolyte complex gel membrane consisting of sodium alginate, cellulose sulphate and poly(methylene-co-guanidine) attached onto a miniaturized Clark oxygen electrode, forming whole cell amperometric biosensor. Measured changes in oxygen concentration were proportional to changes in 2-PE concentration. The biosensor sensitivity was 864nAmM<SUP>-1</SUP> (RSD=6%), a detection limit of 1&mu;M, and the biosensor response towards 2-PE was linear in the range 0.02-0.70mM. The biosensor preserved 93% of its initial sensitivity after 7h of continuous operation and exhibited excellent storage stability with loss of only 6% of initial sensitivity within two months, when stored at 4<SUP>o</SUP>C. The developed system was designed and successfully used for an off-line monitoring of whole course of 2-PE biooxidation process producing phenylacetic acid (PA) as industrially valuable aromatic compound. The biosensor measurement did not require the use of hazardous organic solvent. The biosensor response to 2-PE was not affected by interferences from PA and phenylacetaldehyde at concentrations present in real samples during the biotransformation and the results were in a very good agreement with those obtained via gas chromatography.

2015

Elsevier Pub. Co

0003-2670

1873-4324

854

pp.140-144

10.1016/j.aca.2014.11.012

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70966873

Gluconobacter oxydans; Immobilization; 2-Phenylethanol; Phenylacetic acid; Electrochemistry; Biosensor