BioChem - DOS

Metabolic engineering of Clostridium cellulolyticum for production of isobutanol from cellulose

Applied and environmental microbiology

Higashide, Wendy; Li, Yongchao; Yang, Yunfeng; Liao, James C.

<P><B>ABSTRACT</B><P> Producing biofuels directly from cellulose, known as consolidated bioprocessing, is believed to reduce costs substantially compared to a process in which cellulose degradation and fermentation to fuel are accomplished in separate steps. Here we present a metabolic engineering example for the development of a Clostridium cellulolyticum strain for isobutanol synthesis directly from cellulose. This strategy exploits the host's natural cellulolytic activity and the amino acid biosynthesis pathway and diverts its 2-keto acid intermediates toward alcohol synthesis. Specifically, we have demonstrated the first production of isobutanol to approximately 660 mg/liter from crystalline cellulose by using this microorganism. </P></P>

2011

American Society for Microbiology

0099-2240

1098-5336

77

8

pp.2727-2733

10.1128/aem.02335-12

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56415723

https://aem.asm.org/content/aem/78/19/7171.full.pdf