BioChem - DOS

Detection of α-Galactosidase A Reaction in Samples Extracted from Dried Blood Spots Using Ion-Sensitive Field Effect Transistors

Sensors

Kuznetsov, Alexander; Sheshil, Andrey; Smolin, Eugene; Grudtsov, Vitaliy; Ryazantsev, Dmitriy; Shustinskiy, Mark; Tikhonova, Tatiana; Kitiashvili, Irakli; Vechorko, Valerii; Komarova, Natalia

<P>Fabry disease is a lysosomal storage disorder caused by a significant decrease in the activity or absence of the enzyme &alpha;-galactosidase A. The diagnostics of Fabry disease during newborn screening are reasonable, due to the availability of enzyme replacement therapy. This paper presents an electrochemical method using complementary metal-oxide semiconductor (CMOS)-compatible ion-sensitive field effect transistors (ISFETs) with hafnium oxide-sensitive surfaces for the detection of &alpha;-galactosidase A activity in dried blood spot extracts. The capability of ISFETs to detect the reaction catalyzed by &alpha;-galactosidase A was demonstrated. The buffer composition was optimized to provide suitable conditions for both enzyme and ISFET performance. The use of ISFET structures as sensor elements allowed for the label-free detection of enzymatic reactions with melibiose, a natural substrate of &alpha;-galactosidase A, instead of a synthetic fluorogenic one. ISFET chips were packaged with printed circuit boards and microfluidic reaction chambers to enable long-term signal measurement using a custom device. The packaged sensors were demonstrated to discriminate between normal and inhibited GLA activity in dried blood spots extracts. The described method offers a promising solution for increasing the widespread distribution of newborn screening of Fabry disease.</P>

2024

MDPI AG

1424-8220

24

11

pp.3681

10.3390/s24113681

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART130664164