BioChem - DOS

Efficient utilization of cassava pulp for succinate production by metabolically engineered Escherichia coli KJ122

Bioprocess and biosystems engineering

Sawisit, Apichai; Jantama, Sirima Suvarnakuta; Kanchanatawee, Sunthorn; Jantama, Kaemwich

<P>A metabolically engineered Escherichia coli KJ122 was efficiently utilized for succinate production from cassava pulp during batch separate hydrolysis and fermentation (SHF) under simple anaerobic conditions. Succinate concentration of 41.46 &#177; 0.05 g/L with yield and productivity of 82.33 &#177; 0.14 g/100 g dry pulp and 0.84 &#177; 0.02 g/L/h was obtained. In batch simultaneous saccharification and fermentation (SSF), hydrolysis of 12 % (w/v) cassava pulp with an enzyme loading of 2 % AMG + 3 % Cel (v/w) at pH 6.5 was optimized at 39 &#176;C. Succinate concentration of 80.86 &#177; 0.49 g/L with a yield of 70.34 &#177; 0.37 g/100 g dry pulp and a productivity of 0.84 &#177; 0.01 g/L/h was attained using E. coli KJ122. Fed-batch SSF significantly enhanced succinate concentration to 98.63 &#177; 0.12 g/L at yield and productivity of 71.64 &#177; 0.97 g/100 g dry pulp and 1.03 &#177; 0.01 g/L/h. This result indicated an efficient and economical succinate production from cassava pulp using SHF and SSF by the use of E. coli KJ122.</P>

2015

Springer-Verlag

1615-7591

1615-7605

38

1

pp.175-187

10.1007/s00449-014-1257-7

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71402216